Name Henry Miziorko Ph.D.
Marion Merrell Dow Professor
The Miziorko lab investigates enzyme function and catalytic/regulatory mechanisms. Currently, efforts focus on enzymes in the pathway of polyisoprenoid and sterol biosynthesis. The lab has developed a variety of mechanistic tools (spectroscopically detectable substrate analogs, inhibitors, etc.) that are useful for studying these proteins and employs a variety of experimental approaches including: protein engineering/mutagenesis, bioorganic chemistry, biophysical chemistry, mechanistic enzymology, and collaborative structural work. For example, the lab has published a variety of studies on HMG-CoA synthase and mevalonate kinase. Recent work on HMG-CoA synthase has documented active site residues involved in reaction chemistry. The prokaryotic enzyme and a reaction intermediate have been crystallized in our lab and the structures have been solved in collaboration with Prof. David Harrison. Our work on recombinant human, rat, and bacterial mevalonate kinases includes the functional assignment of several active site residues as well as elucidation of the structure of an enzyme-Mg-ATP complex in collaboration with Prof. Jung-Ja Kim.
Biological Sciences Building, Rm 505
Personal Web Page
St. Joseph’s University (Philadelphia, Pa.), B.S., Chemistry
University of Pennsylvania, Ph.D., Biochemistry
School of Biological Sciences
Enzyme regulation and catalysis